Millennial Scientific is pleased to announce a major achievement in our product development: the successful completion of the beta testing phase of NanoPak-C All Carbon analytical grade media. As part of this testing, we conducted a brief study, which we have presented as a white paper. The study outlines a method for the analytical HPLC of Semaglutide and Liraglutide using analytical-grade NanoPak-C All Carbon microbeads under alkaline conditions. Additionally, we compared the HPLC separation results under identical conditions using two commercially available silica C18 media.
The combined results demonstrate that NanoPak-C analytical grade media can effectively separate Semaglutide and Liraglutide with good chromatography resolution. The key chromatography parameters of NanoPak-C are consistent with the expected values for 4-7 µm media. These analytical columns can be used for quality testing of impurities or method development. Moreover, during peptide manufacturing, the 40 µm NanoPak-C media can serve as a cleanup method for Semaglutide and Liraglutide, or as a pre-purification step to initially reduce sample complexity and remove a significant portion of impurities. This cleanup process can be followed by final purification using 5 µm analytical or 10 µm preparative grade media. This workflow would enable straightforward scale-up procedures, ensuring consistent results at the manufacturing scale.
Download our application note on the Separation of Semaglutide and Liraglutide on the NanoPak-C All Carbon HPLC column.
Please contact us to learn more about the Nanopak-C graphitic carbon media and how it can enhance your chromatography, small molecule or large! Please email us at inquiry@millennialscientific.com, call us at 855 388 2800, or fill in our online form.
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