Bovine Serum Albumin (BSA) is a widely used protein standard in various biochemical experiments. Its stability, minimal impact on biochemical reactions, and low cost also make it a suitable protein model for developing pharmaceutical formulations and delivery systems. Thus, robust methods for BSA characterization and quantification are beneficial and essential.
Several analytical methods have been developed and published for characterizing and quantifying BSA. Bicinchoninic acid (BCA) assay and size-exclusion chromatography (SE-HPLC) method are the most common techniques. However, these methods exhibit limitations. Some common pharmaceutical excipients could interfere and generate measurement errors.
Reverse-phase HPLC (High-Performance Liquid Chromatography) is a powerful and well-established technique for separating and analyzing protein mixtures where protein denaturation and loss of biological activity due to the addition of organic modifiers are not an issue.
NanoPak-C All Carbon media offers a tunable pore size and surface area suitable for reverse-phase separation of proteins. BSA’s hydrophobic region can interact favorably with the hydrophobic carbon surface, allowing better protein conformation and stability during chromatography.
This note summarizes the optimal HPLC method for detecting and purifying BSA. The technique uses a UV detector, available in most analytical labs.
Download our application note on Bovine Serum Albumin (BSA) using NanoPak-C All Carbon HPLC column
Get in touch to discuss how we can support your BSA analysis Please email us at inquiry@millennialscientific.com, call us at 855 388 2800 or fill in our online form.
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